FlowVision is offline FCS analysis software for Windows.
Polygon / rect / ellipse / quadrant gates, spillover compensation, biexponential scales, FCS 3.1 — for $99 lifetime, no subscription.
No account required · No credit card · Windows 10 / 11
Built for the routine flow cytometry workflow on Windows. Open FCS, gate, compensate, batch-analyze, report.
FlowJo® is a registered trademark of Becton, Dickinson and Company. FlowVision is independent software, not affiliated with, endorsed by, or sponsored by BD.
No cloud, no account, no telemetry. Your patient samples and unpublished data never touch the internet. Suitable for GLP/GMP and HIPAA-adjacent workflows.
$99 once. You own the version forever, no annual renewals. New major versions are optional paid upgrades when you choose.
FlowVision reads FCS 2.0 / 3.0 / 3.1 / 3.2 and writes standard FCS 3.1. Compensated and unmixed files open in FlowJo, Kaluza, FCS Express. And your gates export to FlowJo .wsp and Gating-ML 2.0 — continue the same analysis in FlowJo or R (flowWorkspace). Your data and your gating stay portable, both directions.
Email info@flowvision.io reaches the developer directly. No support tier escalation, no ticket system, just a reply.
Every feature unlocked. No account, no credit card. Open your FCS files in 2 minutes and decide for yourself.
Download FlowVision for WindowsWindows 10 / 11 (64-bit) · 102 MB installer · Signed by FlowVision
Yes — both ways. FlowVision imports a FlowJo .wsp to read its spillover / compensation matrix (with truncated-file recovery), and it exports your gates back to a FlowJo .wsp — double-click and FlowJo opens the sample with your populations on the correct axes. You can also export to Gating-ML 2.0 (the ISAC open standard) which reads in R (flowWorkspace / CytoML) and FCS Express. Gate in FlowVision when the shared FlowJo license is busy, then hand the .wsp back when it is your turn — a bridge, not a lock-in. See the full FlowJo & R export workflow →
Yes. FlowVision auto-reads the $SPILL / $SPILLOVER keyword from your FCS file (BD FACSDiva and Beckman Kaluza both write this). The matrix inversion uses the same Sᵀ⁻¹ × M model as FlowJo, so compensated populations match within manual gating variance (~1% on typical lymphocyte gates).
FlowVision uses the standard point-in-polygon algorithm (Crossing Number, Gating-ML 2.0 spec) and the biexponential scale published by Parks 2006 and Moore 2012 — the same math used by most flow cytometry tools. Differences come from where you place vertices, not from the algorithms.
Yes. FlowVision parses FCS 2.0, 3.0, 3.1 and 3.2 files from BD (including FACSDiscover S8), Beckman Coulter (including CytoFLEX), Cytek (Aurora), Sony (ID7000) and Stratedigm. The instrument writes the FCS file — FlowVision just reads the standard format.
FlowVision includes UMAP dimensionality reduction built in — with a reproducible seed, distance-metric choice and landmark mode for 20k+ event spectral panels. viSNE, FlowSOM and SeqGeq are not included; if your workflow depends on those specific plugins, FlowJo with paid plugins is still the right tool. FlowVision is built for routine and high-parameter gating + MFI + batch analysis, with UMAP for exploratory structure.
Mac desktop build (Apple Silicon + Intel) ships in v1.7.1. The engine, file format, and workflow are identical to the Windows version. Download directly from flowvision.io.
Try the 15-day free trial first — every feature unlocked. If you decide to buy, there's a 30-day money-back guarantee. Your FCS files stay in standard format, so you can switch back to FlowJo any time without losing data. The risk to try FlowVision is zero.